Websites using sequencing primers design as a keyword

Validated Pipeline for Primer Design and in-silico PCR Submit positions to v0 Primer Design Pipeline (first generation) v1 Primer Design Pipeline v2 Primer Design Pipeline (input your own sequence with SNPs) Open-sourced and published tools for generating and in silico testing of primers designed for deep sequencing (validation) of single nucleotide positions on the Illumina MiSeq Sequencer. The workflow that this primer design pipeline automates is part of our current SOP and is currently in routine laboratory use (see publications in references). Please familiarize yourself with the workflow before submission to the Pipeline. For the various primer3 settings used in the pipeline, please see here.

OLIGO performs a range of functions for researchers in PCR and related technologies, enables to design consensus, multiplex and degenerate primers. Reverse translation, restriction enzyme and open reading frames analysis, oligonucleotide database, primer secondary structure, LCR, siRNA, molecular beacons and nested primers design, real time PCR, batch file processing.